Martin Luther University Halle-Wittenberg

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Research

The development of enzymes for the directed modification and for the downstream processing of (therapeutic) proteins and enzymes are the main research interests of the group of Prof. Pietzsch.

Transglutaminase Kristallstruktur

The previously developed know-how of the design of biocatalysts for the production of optically active low molecular weight compounds (e. g. amino acids) was extended recently to the field of site-directed modification of macromolecules. Research projects focus on the development of a TRANSGLUTAMINASE [Marx, C., Hertel, T. and Pietzsch, M. (2007). Enz. Microb. Technol., 40, 1543 – 1550] by random and site-directed mutagenesis in order to optimize this enzyme, e. g. for the PEGylation of therapeutic proteins. Covalent cross-linking of proteins of animal (collagen, casein) and plant origin in order to produce thin films and foils is another application of this enzyme.  In the research field of downstream processing biocatalysts are investigated for their potential to remove contaminations (e. g. ENDOTOXINS, host cell proteins) specifically. Also, crystallization of proteins for purification purposes and integrated bioprocess development are investigated.

DESIGNER proteins

"Development of protein-based, formaldehyde-free binder"

The aim of the project funded by the European Regional Development Fund (ERDF) is the development of protein-based binders and the biotechnological production of DESIGNER proteins.

DESIGNER proteins are optimized, non-natural proteins. The application of these artificial macromolecules is planned mainly in material science for the development of binders for sand and/or wood materials. Commercially mainly formaldehyde-containing resins are used for this purpose. Because of the disadvantages of these conventional binders in terms of environmental compatibility and health risks, biodegradable alternatives are in the focus of modern science. Our research includes the de novo design of proteins as applicable binders as well as their testing with sand and wood as materials. For the production of the DESIGNER proteins different microbial production strains will be used and their cultivation using renewable resources as carbon source should be optimized.

This project is a contribution to the development of an efficient process for the production of durable protein-based binders that are no risk to environment and health.

Project term: 01.01.2016 - 31.12.2019
Funding period: 01.01.2016 - 31.3.2020


Forschungsportal Sachsen- Anhalt

Here you will find detailed information on research projects of our group.

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