Martin Luther University Halle-Wittenberg

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Methods and Equipement

MethodsEquipment
Cloning and Expression, PCR

Thermocycler, Mol. Biol. Lab.

Cultivation of microorganisms

Bioreactors: 5, 20, 50, 100 L working volume

Purification of enzymes

Cell separation: CEPA Z41 centrifuge
Cell disintegration:

~ homogenizer: McGaulin, Emulsiflex
~ Glass bead mill: Dynomill ~ ultrasound

Concentration: Ultrafiltration: FILTRON

Chromatography: Äkta Explorer, FPLC

Characterization of biocatalysts / Analytics

~ HPLC-ESI-MS, GC, GC-MS
~ MTP Reader: Fluorostar Galaxy
~ Material testing: Zwick 500 N, Zwick ProLine (20 kN), Microscope, UV, etc.

Optimization of biocatalysts

Immobilization; reaction engineering;

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